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y atom sh2 biosensor  (Addgene inc)


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    Structured Review

    Addgene inc y atom sh2 biosensor
    HeLa cells were transfected with one plasmid expressing ER- or mitochondria-localized y-ATOM <t>SH2</t> and a second plasmid encoding either ER- or mitochondrial-targeted SH2 (transfection #1), or SH2 with no localization tags (resulting in cytoplasmic SH2 expression; transfection #2). (A) Cells appeared green when the biosensor and ligand were targeted to the same compartment and were mostly dark when the biosensor was targeted to ER or mitochondria and the ligand was expressed in the cytoplasm. Scale bars are 100 μm. For the quantification at the right side, each dot represents one cell (n ≥ 50), and error bars correspond to the standard deviation. (B) Confocal images of HeLa cells expressing y-ATOM SH2 and SH2 ligand, both targeted to ER (left) or mitochondria (right), reveal the expected mesh-like and tubular structures of the respective organelles. Scale bars are 10 μm.
    Y Atom Sh2 Biosensor, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/y atom sh2 biosensor/product/Addgene inc
    Average 93 stars, based on 1 article reviews
    y atom sh2 biosensor - by Bioz Stars, 2026-04
    93/100 stars

    Images

    1) Product Images from "Sensitive and Adaptable Turn-On Maturation (ATOM) Fluorescent Biosensors for Detecting Subcellular Localization of Protein Targets in Cells"

    Article Title: Sensitive and Adaptable Turn-On Maturation (ATOM) Fluorescent Biosensors for Detecting Subcellular Localization of Protein Targets in Cells

    Journal: Bio-protocol

    doi: 10.21769/BioProtoc.5239

    HeLa cells were transfected with one plasmid expressing ER- or mitochondria-localized y-ATOM SH2 and a second plasmid encoding either ER- or mitochondrial-targeted SH2 (transfection #1), or SH2 with no localization tags (resulting in cytoplasmic SH2 expression; transfection #2). (A) Cells appeared green when the biosensor and ligand were targeted to the same compartment and were mostly dark when the biosensor was targeted to ER or mitochondria and the ligand was expressed in the cytoplasm. Scale bars are 100 μm. For the quantification at the right side, each dot represents one cell (n ≥ 50), and error bars correspond to the standard deviation. (B) Confocal images of HeLa cells expressing y-ATOM SH2 and SH2 ligand, both targeted to ER (left) or mitochondria (right), reveal the expected mesh-like and tubular structures of the respective organelles. Scale bars are 10 μm.
    Figure Legend Snippet: HeLa cells were transfected with one plasmid expressing ER- or mitochondria-localized y-ATOM SH2 and a second plasmid encoding either ER- or mitochondrial-targeted SH2 (transfection #1), or SH2 with no localization tags (resulting in cytoplasmic SH2 expression; transfection #2). (A) Cells appeared green when the biosensor and ligand were targeted to the same compartment and were mostly dark when the biosensor was targeted to ER or mitochondria and the ligand was expressed in the cytoplasm. Scale bars are 100 μm. For the quantification at the right side, each dot represents one cell (n ≥ 50), and error bars correspond to the standard deviation. (B) Confocal images of HeLa cells expressing y-ATOM SH2 and SH2 ligand, both targeted to ER (left) or mitochondria (right), reveal the expected mesh-like and tubular structures of the respective organelles. Scale bars are 10 μm.

    Techniques Used: Transfection, Plasmid Preparation, Expressing, Standard Deviation



    Similar Products

    y atom sh2 biosensor  (Addgene inc)
    93
    Addgene inc y atom sh2 biosensor
    HeLa cells were transfected with one plasmid expressing ER- or mitochondria-localized y-ATOM <t>SH2</t> and a second plasmid encoding either ER- or mitochondrial-targeted SH2 (transfection #1), or SH2 with no localization tags (resulting in cytoplasmic SH2 expression; transfection #2). (A) Cells appeared green when the biosensor and ligand were targeted to the same compartment and were mostly dark when the biosensor was targeted to ER or mitochondria and the ligand was expressed in the cytoplasm. Scale bars are 100 μm. For the quantification at the right side, each dot represents one cell (n ≥ 50), and error bars correspond to the standard deviation. (B) Confocal images of HeLa cells expressing y-ATOM SH2 and SH2 ligand, both targeted to ER (left) or mitochondria (right), reveal the expected mesh-like and tubular structures of the respective organelles. Scale bars are 10 μm.
    Y Atom Sh2 Biosensor, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/y atom sh2 biosensor/product/Addgene inc
    Average 93 stars, based on 1 article reviews
    y atom sh2 biosensor - by Bioz Stars, 2026-04
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    HeLa cells were transfected with one plasmid expressing ER- or mitochondria-localized y-ATOM SH2 and a second plasmid encoding either ER- or mitochondrial-targeted SH2 (transfection #1), or SH2 with no localization tags (resulting in cytoplasmic SH2 expression; transfection #2). (A) Cells appeared green when the biosensor and ligand were targeted to the same compartment and were mostly dark when the biosensor was targeted to ER or mitochondria and the ligand was expressed in the cytoplasm. Scale bars are 100 μm. For the quantification at the right side, each dot represents one cell (n ≥ 50), and error bars correspond to the standard deviation. (B) Confocal images of HeLa cells expressing y-ATOM SH2 and SH2 ligand, both targeted to ER (left) or mitochondria (right), reveal the expected mesh-like and tubular structures of the respective organelles. Scale bars are 10 μm.

    Journal: Bio-protocol

    Article Title: Sensitive and Adaptable Turn-On Maturation (ATOM) Fluorescent Biosensors for Detecting Subcellular Localization of Protein Targets in Cells

    doi: 10.21769/BioProtoc.5239

    Figure Lengend Snippet: HeLa cells were transfected with one plasmid expressing ER- or mitochondria-localized y-ATOM SH2 and a second plasmid encoding either ER- or mitochondrial-targeted SH2 (transfection #1), or SH2 with no localization tags (resulting in cytoplasmic SH2 expression; transfection #2). (A) Cells appeared green when the biosensor and ligand were targeted to the same compartment and were mostly dark when the biosensor was targeted to ER or mitochondria and the ligand was expressed in the cytoplasm. Scale bars are 100 μm. For the quantification at the right side, each dot represents one cell (n ≥ 50), and error bars correspond to the standard deviation. (B) Confocal images of HeLa cells expressing y-ATOM SH2 and SH2 ligand, both targeted to ER (left) or mitochondria (right), reveal the expected mesh-like and tubular structures of the respective organelles. Scale bars are 10 μm.

    Article Snippet: HeLa human epithelial cells (ATCC, catalog number: CCL-2) 4. pCMV-MBP-yATOM_SH2 for expressing y-ATOM SH2 biosensor in mammalian cells (Addgene plasmid # 209708; http://n2t.net/addgene:209708 ; RRID: Addgene_209708) 5. pCMV-MBP-yATOM_WDR5 for expressing y-ATOM WDR5 biosensor in mammalian cells (Addgene plasmid # 209706; http://n2t.net/addgene:209706 ; RRID: Addgene_209706) 6. pCMV-MBP-yATOM_mCherry for expressing y-ATOM mCh biosensor in mammalian cells (Addgene plasmid # 209707; http://n2t.net/addgene:209707 ; RRID: Addgene_209707) 7. pCMV-MBP-yATOM_RAS for expressing y-ATOM RAS biosensor in mammalian cells (Addgene plasmid # 209709; http://n2t.net/addgene:209709 ; RRID: Addgene_209709) 8. pCMV-RBP_WDR5 for expressing WDR5 in mammalian cells (Addgene plasmid # 209702; http://n2t.net/addgene:209702 ; RRID: Addgene_209702) 9. pCMV-mCherry (Y70A) for expressing the Y70A (nonfluorescent) mutant of mCherry in mammalian cells (Addgene plasmid # 209703; http://n2t.net/addgene:209703 ; RRID: Addgene_209703) 10. pCMV-SH2 for expressing SH2 domain in mammalian cells (Addgene plasmid # 209704; http://n2t.net/addgene:209704 ; RRID: Addgene_209704) 11.

    Techniques: Transfection, Plasmid Preparation, Expressing, Standard Deviation